Part:BBa_I50040:Design

nonfunctional pSC101 origin of replication

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Part:pSB4A3 served as the basis for the design of I50040. Two mutations were introduced in order to remove two MfeI sites. These two mutations occur within an inverted repeat and one also occurs within a ribosome binding site. Additional mutations were included to eliminate other potentially useful restriction sites. Thus, the sequence of I50040 differs slightly from that used in Part:pSB4A3. One or more of these mutations rendered I50040 nonfunctional as a replication origin.

Source

This part was synthesized via direct DNA synthesis by Codon Devices, Inc. in Cambridge, MA.

References

<biblio>

1. Cohen-J-Bacteriol-1977 pmid=334752
2. Lutz-Nucleic-Acids-Res-1997 pmid=9092630
3. Elowitz-Nature-2000 pmid=10659856

</biblio>